P-ManNDAz

Product information

• Function

  P-ManNDAz is an unnatural diazirine-containing monosaccharide that acts as a photo-crosslinker. The diazirine group is activated upon UV radiation. ¹

• Mode of action

  P-ManNDAz is taken-up by cells and converted to CMP-SiaDAz inside cells. It is incorporated into cell surface glycoproteins and glycolipids. ¹

• Applications

  P-ManNDAz is a cell-permeable photo-crosslinking probe. It can be used to investigate the role of glycoproteins, glycolipids and their interactions.

• Handling

  P-ManNDAz is soluble in DMSO and PBS and is added to cell culture from a stock solution. Incubation of the cells with P-ManNDAz for 72 hrs leads to detectable levels of Neu5NDAz residues at the cell surface. Sialoglycans decorated with Neu5DAz residues can be photo-crosslinked using 350 nm light for 20 min to achieve a cross-link between the Neu5NDAz modified protein and its binding partner (for example a Siglec receptor). ¹

• Chemical Information

  CAS No.: 1015698-39-1
  SMILES: O=C(CCC1(N=N1)C)N[C@H]2[C@@H](OC(C)=O)[C@H](OC(C)=O)[C@@H](COC(C)=O)OC2OC(C)=O
  Chemical formula: C₁₉H₂₇N₃O₁₀
  Molecular weight: 457.44
  Purity: > 95%
  Identity: ¹H NMR
  Shipping temperature: 20°C
  Storage temperature: -20°C