P-ManNDAz
Metabolic label of sialic acid for photocrosslinking
Product ID: | SV2514 |
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Synonyms: | Ac4ManNDAz, 2-Deoxy-2-[[3-(3-methyl-3H-diazirin-3-yl)-1-oxopropyl]amino]-D-mannopyranose-1,3,4,6-tetraacetate |
Tags: | Diazirine, N-Acetyl-Mannosamine, Neuraminic acid, Photocrosslinker, Sialic acid |
![P-ManNDAz](https://synvenio.com/wp-content/uploads/2020/08/P-ManNDAz.png)
Product | Price | Estimated Shipping Time | Purchase |
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€239.00 | 1-3 days | |
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€329.00 | 1-3 days | |
|
€699.00 | 1-3 days |
Product information
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Function
P-ManNDAz is an unnatural diazirine-containing monosaccharide that acts as a photo-crosslinker. The diazirine group is activated upon UV radiation. 1
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Mode of action
P-ManNDAz is taken-up by cells and converted to CMP-SiaDAz inside cells. It is incorporated into cell surface glycoproteins and glycolipids. 1
![](https://synvenio.com/wp-content/uploads/2020/08/P-ManNDAz-website.png)
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Applications
P-ManNDAz is a cell-permeable photo-crosslinking probe. It can be used to investigate the role of glycoproteins, glycolipids and their interactions.
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Handling
P-ManNDAz is soluble in DMSO and PBS and is added to cell culture from a stock solution. Incubation of the cells with P-ManNDAz for 72 hrs leads to detectable levels of Neu5NDAz residues at the cell surface. Sialoglycans decorated with Neu5DAz residues can be photo-crosslinked using 350 nm light for 20 min to achieve a cross-link between the Neu5NDAz modified protein and its binding partner (for example a Siglec receptor). 1
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Chemical Information
CAS No.: 1015698-39-1
SMILES: O=C(CCC1(N=N1)C)N[C@H]2[C@@H](OC(C)=O)[C@H](OC(C)=O)[C@@H](COC(C)=O)OC2OC(C)=O
Chemical formula: C19H27N3O10
Molecular weight: 457.44
Purity: > 95%
Identity: 1H NMR
Shipping temperature: 20°C
Storage temperature: -20°C
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