Control compound for experiments utilizing mannosamine derivatives
|Tags:||Control compound, N-Acetyl-Mannosamine|
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Mode of action
P-ManNAc is taken up by cells via passive diffusion because this compound contains O-acetyl (OAc) groups. These groups make the molecule more lipophilic so that it can passively diffuse over mammalian cell membranes. Inside cells, these O-acetyl groups are removed via non-specific esterase releasing N-acetylmannosamine and acetic acid. It is possible that at high concentrations, the released acetic acid or the partially acetylated intermediates are toxic. In addition, at high concentrations, non-specific reactions of acetylated precursors and cysteine residues have been observed.2 At low µM concentrations, these effects may be negligible but it is advisable to use a control compounds such as P-ManNAc. Finally, P-ManNAc may be used to boost intracellular ManNAc levels. It has for example been shown that P-Neu5Ac can be used to boost intracellular neuraminic acid levels.3
P-ManNAc is soluble in a mixture of DMSO and PBS and is added to cell culture from a stock solution.
CAS No.: 6730-10-5
Chemical formula: C16H23NO10
Molecular weight: 389.35
Purity: > 95%
Identity: 1H NMR
Shipping temperature: 20°C
Storage temperature: 20°C
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