Click reagents

Bioorthogonal bioconjugation reagents

Product ID:	SV5753
Synonyms:	Copper Sulfate, Sodium Ascorbate, Tris((1-hydroxy-propyl-1H-1,2,3-triazol-4-yl)methyl)amine
Tags:	Alkyne, Azide, Bioorthogonal chemistry

Product	Price	Estimated Shipping Time	Purchase
Click reagents - for 100 wells in 96 well plates	€125.00	1-3 days	Click reagents quantity
Click reagents - for 1000 wells in 96 well plates	€500.00	1-3 days	Click reagents quantity

Function

Copper Sulfate, Sodium Ascorbate and THPTA can be used to perform the copper-catalyzed azide-alkyne cycloaddition reaction (CuAAC) between an azide and alkyne. Copper Sulfate and Sodium Ascorbate are combined to produce the active Copper(I) catalyst. THPTA is a water soluble ligand that reduces the cytotoxicity of the Copper(I) catalyst.
Mode of action

Copper Sulfate is reacted with Sodium Ascorbate to product the active Copper(I) catalyst. Copper ligand THPTA accelerates the copper-catalyzed azide-alkyne cycloaddition reaction (CuAAC) used to selectively conjugate functional handles to azide or alkyne on live or fixed cells. The copper catalyst reagent is cytotoxic due to the formation of reactive oxygen species (ROS).¹ ² THPTA reduces the time needed for the bioorthogonal labeling step and also allows for a lower concentration of (cytotoxic) copper catalyst. THPTA significantly reduces cytotoxicity the bioorthogonal azide-alkyne click reaction.¹ ³

Applications

Catalyzing the copper-catalyzed azide-alkyne cycloaddition reaction (CuAAC)
Handling

The reagents can be prepared and used according to the following protocols.
For the labeling with BiotinAz using THPTA please use this protocol.
For the labeling with BiotinAlk using THPTA please use this protocol.

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Structure Description Metabolic label of GlcNAc/GalNAc residues Functions Metabolic label Purchase Click reagents quantity
	(endo)-sTCO-COOEt	Trans-cyclooctene (TCO)	Strained trans-cyclooctene with ethyl ester	Click reagents quantity
Structure Description Strained trans-cyclooctene with ethyl ester Functions Trans-cyclooctene (TCO) Purchase Click reagents quantity
	P-Neu5NPoc	Metabolic label	Highly potent metabolic label of sialylation	Click reagents quantity
Structure Description Highly potent metabolic label of sialylation Functions Metabolic label Purchase Click reagents quantity
	P-GalNAc6Az	Metabolic label	Specific metabolic label of intracellular β-O-GlcNAc residues	Click reagents quantity
Structure Description Specific metabolic label of intracellular β-O-GlcNAc residues Functions Metabolic label Purchase Click reagents quantity
	P-GalNPoc	Metabolic label	Metabolic label of GlcNAc/GalNAc residues	Click reagents quantity
Structure Description Metabolic label of GlcNAc/GalNAc residues Functions Metabolic label Purchase Click reagents quantity

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