Click reagents

Bioorthogonal bioconjugation reagents

Product ID:	SV5753
Synonyms:	Copper Sulfate, Sodium Ascorbate, Tris((1-hydroxy-propyl-1H-1,2,3-triazol-4-yl)methyl)amine
Tags:	Alkyne, Azide, Bioorthogonal chemistry

Product	Price	Estimated Shipping Time	Purchase
Click reagents - for 100 wells in 96 well plates	€125.00	1-3 days	Click reagents quantity
Click reagents - for 1000 wells in 96 well plates	€500.00	1-3 days	Click reagents quantity

Function

Copper Sulfate, Sodium Ascorbate and THPTA can be used to perform the copper-catalyzed azide-alkyne cycloaddition reaction (CuAAC) between an azide and alkyne. Copper Sulfate and Sodium Ascorbate are combined to produce the active Copper(I) catalyst. THPTA is a water soluble ligand that reduces the cytotoxicity of the Copper(I) catalyst.
Mode of action

Copper Sulfate is reacted with Sodium Ascorbate to product the active Copper(I) catalyst. Copper ligand THPTA accelerates the copper-catalyzed azide-alkyne cycloaddition reaction (CuAAC) used to selectively conjugate functional handles to azide or alkyne on live or fixed cells. The copper catalyst reagent is cytotoxic due to the formation of reactive oxygen species (ROS).¹ ² THPTA reduces the time needed for the bioorthogonal labeling step and also allows for a lower concentration of (cytotoxic) copper catalyst. THPTA significantly reduces cytotoxicity the bioorthogonal azide-alkyne click reaction.¹ ³

Applications

Catalyzing the copper-catalyzed azide-alkyne cycloaddition reaction (CuAAC)
Handling

The reagents can be prepared and used according to the following protocols.
For the labeling with BiotinAz using THPTA please use this protocol.
For the labeling with BiotinAlk using THPTA please use this protocol.

Structure	Name	Functions	Description	Purchase
	P-GalNPoc	Metabolic label	Metabolic label of GlcNAc/GalNAc residues	1-3 days Click reagents quantity
Structure Description Metabolic label of GlcNAc/GalNAc residues Functions Metabolic label Purchase 1-3 days Click reagents quantity
	P-GlcNAc	Natural monosaccharide	Control compound for experiments utilizing glucosamine derivatives	1-3 days Click reagents quantity
Structure Description Control compound for experiments utilizing glucosamine derivatives Functions Natural monosaccharide Purchase 1-3 days Click reagents quantity
	P-GalNAz	Metabolic label	Metabolic label of GlcNAc/GalNAc residues	1-3 days Click reagents quantity
Structure Description Metabolic label of GlcNAc/GalNAc residues Functions Metabolic label Purchase 1-3 days Click reagents quantity
	P-GlcNPoc	Metabolic label	Metabolic label of GlcNAc residues	1-3 days Click reagents quantity
Structure Description Metabolic label of GlcNAc residues Functions Metabolic label Purchase 1-3 days Click reagents quantity
	P-GlcNAc6Az	Metabolic label	Specific metabolic label of intracellular β-O-GlcNAc residues	1-3 days Click reagents quantity
Structure Description Specific metabolic label of intracellular β-O-GlcNAc residues Functions Metabolic label Purchase 1-3 days Click reagents quantity

Required weight for stock solution
Dissolve the required mass in your desired stock volume.

Stock volume *

Unit *

Stock conc. *

Unit *

Molecular weight of product

Required mass

Unit *

Required volume for dilution
Dilute the required volume of your stock solution to the desired final volume.

Final volume *

Unit *

Stock conc. *

Unit *

Final conc. *

Unit *

Required volume

Unit *

Our Products