Metabolic label of sialic acid for photocrosslinking

Product ID: SV2514
Synonyms: Ac4ManNDAz, 2-Deoxy-2-[[3-(3-methyl-3H-diazirin-3-yl)-1-oxopropyl]amino]-D-mannopyranose-1,3,4,6-tetraacetate
Tags: Diazirine, N-Acetyl-Mannosamine, Neuraminic acid, Photocrosslinker, Sialic acid
Product Price Estimated Shipping Time Purchase
P-ManNDAz - 1 mg
239.00 1-3 days
P-ManNDAz - 10 mg
329.00 1-3 days
P-ManNDAz - 25 mg
699.00 1-3 days

Product information

  • Function

    P-ManNDAz is an unnatural diazirine-containing monosaccharide that acts as a photo-crosslinker. The diazirine group is activated upon UV radiation. 1

  • Mode of action

    P-ManNDAz is taken-up by cells and converted to CMP-SiaDAz inside cells. It is incorporated into cell surface glycoproteins and glycolipids. 1

  • Applications

    P-ManNDAz is a cell-permeable photo-crosslinking probe. It can be used to investigate the role of glycoproteins, glycolipids and their interactions.

  • Handling

    P-ManNDAz is soluble in DMSO and PBS and is added to cell culture from a stock solution. Incubation of the cells with P-ManNDAz for 72 hrs leads to detectable levels of Neu5NDAz residues at the cell surface. Sialoglycans decorated with Neu5DAz residues can be photo-crosslinked using 350 nm light for 20 min to achieve a cross-link between the Neu5NDAz modified protein and its binding partner (for example a Siglec receptor). 1

  • Chemical Information

    CAS No.: 1015698-39-1
    SMILES: O=C(CCC1(N=N1)C)N[C@H]2[C@@H](OC(C)=O)[C@H](OC(C)=O)[C@@H](COC(C)=O)OC2OC(C)=O
    Chemical formula: C19H27N3O10
    Molecular weight: 457.44
    Purity: > 95%
    Identity: 1H NMR
    Shipping temperature: 20°C
    Storage temperature: -20°C


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