BnGalNAc

Product information

• Function

  BnGalNAc is a decoy substrate for the biosynthesis of O-linked glycans based on O-GalNAc. It can be used to inhibit the biosynthesis of membrane linked O-GalNAc containing glycans. Instead cells produce and excrete glycans attached to the BnGalNAc decoy substrate which can be analyzed with mass spectrometry. A more cell permeable and potent derivative of BnGalNAc called P-BnGalNAc is also available here.¹

• Mode of action

  O-linked glycans based on α-O-GalNAc residues starts with the addition of α-O-GalNAc residues to serine or threonine side chains. Next, additional sugars are added to the α-O-GalNAc residue. BnGalNAc is a cell permeable derivative of GalNAc. It enters cells and acts as a decoy substrate. Instead of adding monosaccharides to α-O-GalNAc glycoproteins, these residues are added to BnGalNAc. The corresponding glycoconjugates are excreted into the cell medium and the synthesis of mucins and other glycoconjugates based on α-O-GalNAc is inhibited.

• Applications

  BnGalNAc has been used to study the role of mucins in colorectal cancers.²³

• Handling

  Handling instructions:
  BnGalNAc is soluble in water and PBS and added to cells in from a stock solution. Good priming/inhibition is achieved at 1mM concentration.

• Chemical Information

  CAS No.: 3554-93-6
  SMILES: O[C@@H]1[C@H](O)[C@@H](NC(C)=O)[C@@H](OCC2=CC=CC=C2)O[C@@H]1CO
  Chemical formula: C₁₅H₂₁NO₆
  Molecular weight: 311.33
  Purity: > 95%
  Identity: ¹H NMR
  Shipping temperature: 20°C
  Storage temperature: -20°C